Census of the viral metagenome within an activated sludge microbial assemblage.

نویسندگان

  • Larissa C Parsley
  • Erin J Consuegra
  • Stephen J Thomas
  • Jaysheel Bhavsar
  • Andrew M Land
  • Nadia N Bhuiyan
  • Mustafa A Mazher
  • Robert J Waters
  • K Eric Wommack
  • Willie F Harper
  • Mark R Liles
چکیده

The viral metagenome within an activated sludge microbial assemblage was sampled using culture-dependent and culture-independent methods and compared to the diversity of activated sludge bacterial taxa. A total of 70 unique cultured bacterial isolates, 24 cultured bacteriophages, 829 bacterial metagenomic clones of 16S rRNA genes, and 1,161 viral metagenomic clones were subjected to a phylogenetic analysis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Plasmid Metagenome Reveals High Levels of Antibiotic Resistance Genes and Mobile Genetic Elements in Activated Sludge

The overuse or misuse of antibiotics has accelerated antibiotic resistance, creating a major challenge for the public health in the world. Sewage treatment plants (STPs) are considered as important reservoirs for antibiotic resistance genes (ARGs) and activated sludge characterized with high microbial density and diversity facilitates ARG horizontal gene transfer (HGT) via mobile genetic elemen...

متن کامل

Gene cloning and characterization of a novel esterase from activated sludge metagenome

A metagenomic library was prepared using pCC2FOS vector containing about 3.0 Gbp of community DNA from the microbial assemblage of activated sludge. Screening of a part of the un-amplified library resulted in the finding of 1 unique lipolytic clone capable of hydrolyzing tributyrin, in which an esterase gene was identified. This esterase/lipase gene consists of 834 bp and encodes a polypeptide ...

متن کامل

Experimental Comparison of Two Modifications of Activated Sludge for Treatment of Furfural-Containing Wastewater

 A case study is presented in which two modifications of activated sludge treatment of complex chemical wastewater are experimentally compared: a combination of common activated sludge with powdered activated carbon treatment (PACT), and bioaugmentation of activated sludge treatment (BAST). Industrial wastewater of Pars Oil Refinery that was passed through an oil recovery stage was used to inve...

متن کامل

Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome

BACKGROUND Shotgun metagenomics has become an important tool for investigating the ecology of microorganisms. Underlying these investigations is the assumption that metagenome sequence data accurately estimates the census of microbial populations. Multiple displacement amplification (MDA) of microbial community DNA is often used in cases where it is difficult to obtain enough DNA for sequencing...

متن کامل

Biological Pretreatment of a Beverage Waste Using Yeast Isolated from the Factory Sludge (RESEARCH NOTE)

The beverage industry has been a heavy producer of organic pollution. The major contaminants found in waste are biodegradable organic compounds, volatile organic compounds, toxic metals, and recalcitrant xenobiotics, suspend solids, nutrients (Nitrogen and Phosphor) Microbial pathogens and parasites. Activated sludge flocs contain a wide range of microorganisms such as bacteria, Fungi, yeast, v...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 76 8  شماره 

صفحات  -

تاریخ انتشار 2010